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| Cell line: | MHH-CALL-4 |
| DSMZ no.: | ACC 337 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established from the peripheral blood of a 10-year-old boy with acute lymphoblastic leukemia (pre B-ALL) at diagnosis in 1993 |
| Reference(s): | 15018 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, small round cells growing in suspension, partly in clusters; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/ml (initially in a 24-well-plate is of advantage); optimal split ratio at about 1:2 once a week; culture should not be maintained <0.6 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 80-100 hours |
| Harvest: | cell harvest of ca. 2 x 106 cells/ml |
| Storage: | frozen in serum-free freezing medium (BambankerTM) |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3-, CD10+, CD13+, CD19+, CD20+, CD34-, CD37+, CD38+, CD80-, CD138-, HLA-DR+, cyCD79a+,
cIgG-, cIgM-, cIg-kappa-, cIg-lambda-, sIgG-, sIgM-, sIg-kappa-, sIg-lambda-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 4% polyploidy - 45(42-46)<2n>X, -Y, r(12)(qter;p13-pter) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
