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| Cell line: | MHH-ES-1 |
| DSMZ no.: | ACC 167 |
| Species: | human (Homo sapiens) |
| Cell type: | Ewing's sarcoma |
| Origin: | established from the ascites of a 12-year-old boy with Ewing's sarcoma (type 2) of the left pelvis (with peritoneal metastasis); cells carry EWS-FLI1 fusion gene with t(11;22) |
| Reference(s): | 15155 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells of variable size, single or in small clusters, growing as monolayer; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1.5-2 x 106 cells/80 cm2; split confluent culture 1:3 -1:5 every 2-3 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca 48 hours |
| Harvest: | about 10-15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 12% polyploidy - 49(46-51)<2n>XXY, +X, +1, +8, +12, -17, dup(1)(q31.1q32.2), del(3)(p11), der(4)t(4;?6)(p14;p11), t(6;7)(q11;p11), der(8)t(8;11)(p12;q13), t(11;22)(q24;q12) - presence of type-2 EWS-FLI1 hybrid transcripts (394 bp) confirmed by RT-PCR |
| Molec. Genetics: | expression of fusion gene EWS-FLI1 confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
