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| Cell line: | MHH-TALL-2 |
| DSMZ no.: | ACC 728 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell acute lymphoblastic leukemia |
| Origin: | established in 1994 from the peripheral blood of a boy with T cell acute lymphoblastic leukemia (T-ALL) |
| Reference(s): | 16747 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single cells growing in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1-1.5 x 106 cells/ml in 24-well plates; maintain at 1-3 x 106 cells/ml; split saturated culture 1:2 or 1:3 every 3-4 days; cells are difficult to culture; possibly no cell growth during the first week after thawing; a significant amount of cell debris may be present all the time |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-72 hours |
| Harvest: | maximum density at ca. 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human diploid karyotype with 2% polyploidy; 46(44-46)<2n>XY; no consistent abnormality detected |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
