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| Cell line: | MIA-PACA-2 |
| DSMZ no.: | ACC 733 |
| Species: | human (Homo sapiens) |
| Cell type: | pancreas carcinoma |
| Origin: | established in 1975 from the tumor tissue of a 65-year-old man with undifferentiated pancreatic carcinoma; widely used cell line but described to exhibit neuroendocrine featues (see Cancer Genome Atlas, ref 40712); further described to carry mutations in KRAS, CDKN2A and TP53 |
| Reference(s): | 17196, 40728, 40712 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial cells growing adherently as monolayers and in small clumps; image; image; image |
| Medium: | DMEM + 10% h.i. FBS + 2.5% horse serum |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 5 days using trypsin/EDTA; occasionally, adherent clumps can be detached mechanically |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
