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Cell line: | MKN-45 |
DSMZ no.: | ACC 409 |
Species: | human (Homo sapiens) |
Cell type: | gastric adenocarcinoma |
Origin: | established from the poorly differentiated adenocarcinoma of the stomach (medullary type) of a 62-year-old woman |
Reference(s): | 14790, 14795, 14886 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | both spindle-shaped or oval cells growing in monolayers and single round cells or clumps in suspension; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | cells can be difficult to culture; seed out at ca. 1-2 x 106 cells/80 cm2; split semi-confluent culture 1:3 to 1:5 every 5-10 days using trypsin/EDTA (for 5 min or longer); culture medium may turn acidic (yellow) very quickly, exchange of spent medium is advised, also the cells in suspension may be used to seed a new flask |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 60 hours |
Harvest: | cell harvest of ca. 10-15 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype with 8% polyploidy - 70-73<3n>XX/XXX, -X, -2, -2, -4, -5, -5, +6, -11, -13, -13, -14, -18, -19, -20, -21, -22, +20mar, add(X)(q1?), der(1)(1pter->p21::1p11->q32::1q44->q11::?), i(2p), der(3)t(3;?9)(p14;q13/21), del(4)(q21), del(4)(q26), del(6)(q1?)x2, del(7)(p11), del(8)(p12), add(8)(q24), del(9)(p11), add(13)(p11), inv(16)(p13q22), add(17)(p1?), add(19)(q13), i(21q) - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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