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| Cell line: | MOLM-6 |
| DSMZ no.: | ACC 611 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic myeloid leukemia in blast crisis |
| Origin: | established from the peripheral blood of a 44-year-old man with Philadelphia chromosome-positive chronic myeloid leukemia (CML) in blast crisis in 1992 |
| Reference(s): | 15023 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round cells in suspension; some cells may be adherent; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 1-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 twice a week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48-72 hours |
| Harvest: | cell harvest of ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat moded near triploid karyotype; 62-77<3n>XY/XXY, +6, +6, -14, -15, -16, -17, -18, +22, +22, +2mar, t(9;22)(q34;q11), i(11q), der(22)t(9;22)(q34;q11)x3; add(7)(p11) present in sdl; cells typically carry 4 copies of der(22)t(9;22) / Ph effecting BCR-ABL1 rearrangement |
| Molec. Genetics: | expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
