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Cell line: | MOLP-8 |
DSMZ no.: | ACC 569 |
Species: | human (Homo sapiens) |
Cell type: | multiple myeloma |
Origin: | established from the peripheral blood of a 52-year-old man with multiple myeloma (stage IIIA, type IgD lambda) in 2002 |
Reference(s): | 14748 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single round cells in suspension, some cells loosely adherent; image; image;
image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; max. density at about 1.5 x 106 cells/ml; cells should be grown in 24- or 12-well-plates; immediately after thawing viability may drop to 50%, but cells recover within a few days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40 hours |
Harvest: | cell harvest of ca. 1 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded near-diploid karyotype - 40-48<2n>X, -Y, -15, +1-2mar, t(3;8)(p22;q22), der(4)t(4;15)(q32;q21), inv(8)(q21q24), der(11)t(?4 ;11)(q31;q13), t(11;14)(q13;q32), t(16;18)(p13;q21), der(17)t(11;17)(q13;p13) - carries t(11;14) effecting IGH-CCND1 juxtaposition with additional MYC rearrangement present in inv(8) - matches published karyotype |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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