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| Cell line: | MUTZ-5 |
| DSMZ no.: | ACC 490 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established in 1998 from the peripheral blood of a 26-year-old man with B cell precursor acute lymphoblastic leukemia (BCP-ALL) at relapse |
| Reference(s): | 14762, 40437 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | cells grow singly or in small clusters in suspension, irregular cell forms; image; image |
| Medium: | 80% McCoy's 5A + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 1 x 106 cells/ml; after thawing, culturing in 24-well-plate is of advantage; split saturated culture 1:2 to 1:3 every 2-4 days; minimum cell density of 0.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70-80 hours |
| Harvest: | maximum density at 3.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3-, CD10+, CD13-, CD19+, CD20+, CD30+, CD33-, CD34(+), CD37+, CD38+, CD71+, CD80-, CD138-, HLA-DR+, cyCD79a-, sIgG-, cIgG-, sIgM-, cIgM-, sIg-kappa-, cIg-kappa-, sIg-lambda-, sIg-kappa-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype - 45<2n>X, -Y, t(12;13)(p12;q13-14) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
