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| Cell line: | MUTZ-8 |
| DSMZ no.: | ACC 689 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established in 1994 from the peripheral blood of a 63-year-old woman with secondary acute myelomonocytic leukemia (sAML M4) 25 years after onset of myelodysplastic syndrom (MDS) and 2 years after chemotherapy with Ara-C; cells are constitutively cytokine-dependent
were described in the literature to carry JAK2 and TP53 mutations and a deletion of the 5q31 AML/MDS region effected by a t(5;11) translocation |
| Reference(s): | 16047, 16046, 14871 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorph cells growing singly in suspension with a few cell clusters; occassionally giant cells can be observed; image; image; image; image |
| Medium: | 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 10 ng/ml GM-CSF or 10% vol conditioned medium of cell line 5637 (DSMZ ACC 35) |
| Subculture: | seed out at ca. 0.8 x 106 cells/ml in 24-well plates; maintain at ca. 0.5-1.0 x 106 cells/ml; split culture 1:2 once or twice a week; after thawing cells may need some days to recover and a certain amount of cell debris may be allways visible; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 3-5 days |
| Harvest: | maximum density at ca. 1.2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD4 -, CD13 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertriploid/hypotetraploid karyotype; 71-92<4n>XXXX, -7, -7, -8, -9, -11, -12, -14, der(5)t(5;11)(q21;q10)x2, der(11;12)(p10;q10), der(17)t(10;17)(q11;q24)?dup(17)(p13pter)x2; matches published karyotype |
| Molec. Genetics: | expression of mutated JAK2 confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
