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| Cell line: | Mac-2B |
| DSMZ no.: | ACC 992 |
| Species: | human (Homo sapiens) |
| Cell type: | cutaneous T-cell lymphoma |
| Origin: | established in 1987 from an ulcerating skin lesion of a 47-year-old man with cutaneous large-cell T-cell lymphoma (CTCL) and 12 years after the initial diagnosis with Hodgkin lymphoma of mixed cellularity. Cell line carries translocation t(8;9)(p22;p24) and numerous additional chromosomal abnormalities. Serial sister cell line of Mac-1 (ACC 990), which was established 2-years earlier, and simultaneous sister cell line of Mac-2A (ACC 991). Sister cell lines differ in karyotypes and immunophenotypes |
| Reference(s): | 41008, 41007, 41005, 41006 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | mainly round cells growing as single cells and small clusters in suspension with a lot of "spikes", few larger cells; image; image; image |
| Medium: | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture: | seed out at ca. 0.1-0.4 x 106 cells/ml in 6- to 24-well plate; split 1:2 to 1:8 every 2-4 days; maintain at ca. 0.1-0.6 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 24-50 hours (depending on concentration of FBS and cell density) |
| Harvest: | maximum density at about 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD2-, CD3-, CD4-, CD5-, CD6-, CD7-, CD8(+), CD13-, CD15+, CD16-, CD19-, CD30+, CD34-, TCRalpha/beta-, TCRgamma/delta-, cyCD3-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to that of Mac-1 and Mac-2A |
| Species validation: | human |
| Cytogenetics: | human hypodiploid karyotype -
45<2n> X, -Y, del(1)(p31p32), der(3)t(3;3)(p25;q11), del(6)(q15), t(8;9)(p22;p24), der(10)t(6;10)(q11;p11), del(12)(q11q14), der(15)t(2;15)(p11;p11) |
| Molec. Genetics: | expression of fusion gene PCM1::JAK2 confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
