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Cell line: | N18TG2 |
DSMZ no.: | ACC 103 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | neuroblastoma |
Origin: | 6-thioguanine resistant mutant of cell line N18 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described in the literature to form cAMP in response to prostaglandin E1 and to be aneuploid (modal chromosome number of 81) |
Reference(s): | 14598, 14766, 14355 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round, single, slightly adherent cells, occasionally with short processes; image; image |
Medium: | 90% DMEM + 10% h.i. FBS + 100 µM 6-thioguanine |
Subculture: | split culture 1:5 to 1:10 every 3-5 days (cells grow adherent, but can be detached with trypsin); seed out at 1 x 106 cells/80 cm2 |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 40 hours |
Harvest: | about 1.5 x 107 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
Supplied as: | see price list |
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