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Cell line: | N4TG3 |
DSMZ no.: | ACC 101 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | neuroblastoma |
Origin: | 6-thioguanine resistant mutant of cell line N4 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described in the literature to form increased levels of cAMP in response to prostaglandin E1 which is inhibited by opiate receptors; cells are aneuploid (modal chromosome number of 86) |
Reference(s): | 14355, 15020 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | flat cell body, slightly adherent, occasionally with short processes; image; image; image; image; image |
Medium: | 90% DMEM + 10% h.i. FBS + 100 µM 6-thioguanine |
Subculture: | split saturated culture 1:2 to 1:4 every 2-4 days (cells grow slightly adherent, but can be detached with trypsin) |
Incubation: | at 37 °C with 10% CO2 |
Harvest: | about 10-20 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Viruses: | ELISA: reverse transcriptase positive |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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