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| Cell line: | NALM-20 |
| DSMZ no.: | ACC 681 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established in 1989 from the peripheral blood of a 62-year-old man at diagnosis of acute undifferentiated leukemia (AUL); ; cells were described in the literature to carry a Philadelphia chromosome t(9;22) with a BCR-ABL1 e1-a2 fusion gene; the cell line is a serial sister cell line of NALM-21 (ACC 682) |
| Reference(s): | 15848, 18261 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small round cells growing singly or occasionally in clumps; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.8-1.5 x 106 cells/ml in 24-well plates; maintain at ca. 0.6-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-5 days; cells grow slowly after recovery from liquid nitrogen |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-80 hours |
| Harvest: | cell harvest of ca. 2.5-3.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 +, CD19 +, CD38 +, cyCD79a -, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype; 48(45-48)<2n>XY, +8, +8, inv(4)(p14q35), del(8)(p12)x2, t(9;22)(q34;q11); carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype |
| Molec. Genetics: | expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
