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established in 1989 from the peripheral blood of a 62-year-old man at diagnosis of acute undifferentiated leukemia (AUL); ; cells were described in the literature to carry a Philadelphia chromosome t(9;22) with a BCR-ABL1 e1-a2 fusion gene; the cell line is a serial sister cell line of NALM-21 (ACC 682)
small round cells growing singly or occasionally in clumps; image; image
80% RPMI 1640 + 20% h.i. FBS
seed out at ca. 0.8-1.5 x 106 cells/ml in 24-well plates; maintain at ca. 0.6-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-5 days; cells grow slowly after recovery from liquid nitrogen