NALM-33

Product number: ACC 782

from 140,00 €

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Cell line:

NALM-33

DSMZ no.:

ACC 782

Species:

human (Homo sapiens)

Cell type:

B cell precursor leukemia

Origin:

established in 1996 from the peripheral blood of a 72-year-old man with B cell precursor (BCP) acute lymphoblastic leukemia (ALL) at relapse

Reference(s):

Biosafety level:

Permissions and restrictions:

DSMZ Cell Culture Data:

Morphology:

single round cells growing in suspension

Medium:

80-90% RPMI 1640 + 10-20% h.i. FBS

Subculture:

seed out at ca. 1 x 10⁶ cells/ml in 24-well plates, maintain at 1-1.5 x 10(6) cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 48 hours

Harvest:

maximum density at ca. 2.5-3 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in PCR assay

Immunology:

CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €