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| Cell line: | NCI-H510A |
| DSMZ no.: | ACC 568 |
| Species: | human (Homo sapiens) |
| Cell type: | small cell lung carcinoma |
| Origin: | established in 1982 from an adrenal gland metastasis of a small cell lung cancer from a man in relapse following treatment; cells were described in the literature to be tumorigenic in nude mice and to express elevated levels of L-dopa decarboxylase, neuron-specific enolase, creatine kinase and bombesin-like immunoreactivity; listed in the CCLE (ref 18232) with genome and protein analyses |
| Reference(s): | 14421, 14849, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells growing in monolayers or in clumps in suspension; image; image; image |
| Medium: | 90% RPMI 1640 + Ham's F12 (at 1:1) + 10% h.i. FBS |
| Subculture: | split confluent culture 1:2 once a week using trypsin-like enzyme (e.g. Tryple Express) (standard trypsin/EDTA is not sufficiently effective); seed out 2-3 x 106 cells in 25 cm2; note that certain plastic flasks from some suppliers are inhibitory to growth, while others are conducive |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 1 week |
| Harvest: | cell harvest of ca. 40 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
