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Cell line: | NEURO-2A |
DSMZ no.: | ACC 148 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | neuroblastoma |
Origin: | established from the spontaneous tumor of a strain A albino mouse |
Reference(s): | 14667, 14826 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | about 30% of the cells grow like neuronal cells; 70% are round, loosely attached cells; image; image |
Medium: | 90% DMEM + 10% h.i. FBS + 1x non-essential amino acids |
Subculture: | seed out at ca. 1 x 106 cells/25 cm2; split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 70 hours |
Harvest: | about 8 x 106 cells/75 cm2; maximal density at ca. 20 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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