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Cell line: | NUC-5 |
DSMZ no.: | ACC 226 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | urinary bladder |
Origin: | established from an adult female C3H/Law mouse; non-tumorigenic in vitro immortalized urine bladder cell line with aberrant growth factor response, aneuploid (chromosome number 70-75) |
Reference(s): | 14449 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | large and flat regularly arranged cells, growing in up to 3 cell layers; image; image; image |
Medium: | 45% Ham's F10 + 45% DMEM + 10% h.i. FBS + 5 µg/ml human insulin + 5 µg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite + 10 µM HEPES |
Subculture: | split confluent culture 1:4 every 6-7 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 60-75 hours |
Harvest: | harvest of 2-3 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine hypotetraploid karyotype with 14% polyploidy and 1-3 dmin in 34% cells - 70(64-74)<4n> - 1-3 centric fusion markers present - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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