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Cell line: | OAC-M5.1 |
DSMZ no.: | ACC 696 |
Species: | human (Homo sapiens) |
Cell type: | esophageal adenocarcinoma |
Origin: | established from a lymph node metastasis of a 47-year-old white woman with esophageal adenocarcinoma in 1996; whole genome sequence data in ref 40877 |
Reference(s): | 16054, 16055, 40877 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | polymorphic cells growing lightly adherent and in clumps in suspension |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 0.3 x 106 cells/ml; maintain at ca. 0.2-0.5 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; adherent cells can be detached by pipetting or tapping |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | cell harvest of ca. 0.8 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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