| |
---|
Cell line: | OCI-AML4 |
DSMZ no.: | ACC 729 |
Species: | human (Homo sapiens) |
Cell type: | acute myeloid leukemia |
Origin: | established in 1987 from the blast cells of the peripheral blood of a 35-year-old woman with acute myeloblastic leukemia (AML M4), secondary to the treatment of nodular sclerosing Hodgkin disease with radiation and chemotherapy; cells were described in the literature to grow slowly with stimulation but responds to IL-3, GM-CSF and mast cell growth factor (MGF); cells exhibit wild-type TP53 |
Reference(s): | 16846, 16847 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | round to polymorphic cells growing singly or in clumps in suspension, some cells are adherent; image; image; image; image; image |
Medium: | 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.5 x 106 cells/ml; optimal split ratio 1:2 to 1:4 every 2-4 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 24-30 hours |
Harvest: | maximum density at ca. 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD33 +, HLA-DR +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat moded near diploid karyotype with 3% polyploidy; -46(43-47)<2n>XX, der(6)(3qter->3q27::3q26->3q25::6p21->6q27::3q26->3q27::3q26->3qter), der(11)t(11;19)(q23;p13.3), der(16)t(1;16)(q22;p13), der(18)dup(18)(q11q23.2)t(X;18)(p22;q23.3), der(19)t(8;19)(q12;p13.1), der(22)t(11;22)(q23;q22)t(11;19)(q23;p13.3); carries semi-cryptic t(11;19) effecting rearrangement of KMT2A (MLL) with MLLT1 (ENL) |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |