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| Cell line: | OCI-LY19 |
| DSMZ no.: | ACC 528 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the bone marrow of a 27-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL) (diffuse large cell lymphoma, DLCL, stage 4B, at relapse) in 1987. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s): | 14427, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round cells growing in suspension; image; image; image |
| Medium: | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:4 to 1:6 every 3-5 days; maintain at ca. 0.5-2.0 x 106 cells/ml; one day after thawing the viability may drop down to 50%, but cells recover within one week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40 hours |
| Harvest: | cell harvest of ca. 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD10+, CD13-, CD19+, CD20(+), CD34(+), CD37-, CD38+, CD80-, CD138-, HLA-DR(+), sIgG+, sIgM-, cIg-kappa-, sIg-lambda+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy - 48(46-52)<2n>X, -X, +6, +6, +8, t(4;8)(q3?2;q?24), del(6)(q15)x2, r(8)(??), t(14;18)(q32;q21), add(18)(q23) - carries t(14;18) effecting IGH-BCL2 juxtaposition |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
