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Cell line: | OCI-LY3 |
DSMZ no.: | ACC 761 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established in 1983 from the bone marrow sample of a 52-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large B-cell lymphoma, DLBCL, stage 4B) at relapse; cells were described in the literature to be EBV-negative and lacking a classical t(8;14) translocation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
Reference(s): | 14427, 18975, 15976, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly or in clumps in suspension; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 0.2-0.3 x 106 cells/ml, at best in a 12- or 24-well plate; after thawing cell viability may be as low as 50%, but cells should recover quickly within a week; later, maintain at ca. 0.1-0.8 x 106 cells/ml and
split 1:3 - 1:5 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 24 hours |
Harvest: | maximum density at ca. 0.9 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 (+), CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; in the referenced paper this cell line is described as cykappa +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypertriploid karyotype; 72-77<3n>XXYY, +1, +9, -10, +13, +14, -17, +19, +20, +22, der(1)t(1;17)(p13;q12)x2, der(4)t(4;18)(q31;q21)x2, del(6)(q13)x2, der(6)t(6;6)(p24;q12), der(7)t(6;7)(p24;p22), der(14)t(14;19)(q32;q13.3)x2, del(18)(q21), der(19)t(4;19)(q21;q13)t(4;18)(q31;q21)x2, der(19)t(14;19), dup(20)(q11q13)x2; sdl with der(6)t(6;12)(p21;q21), der(7)t(5;7)(?p15;p24) etc; resembles published karyotypes; carries cryptic t(14;19) with rearrangement of IGH and SPIB, and t(4;18) with copy number amplification of the BCL2 region |
Viruses: | PCR: HBV -, HCV -, HIV-1 -, HIV-2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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