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| Cell line: | OCI-LY7 |
| DSMZ no.: | ACC 688 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the peripheral blood sample of a 48-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large B-cell lymphoma, DLBCL, stage 2A, at relapse) in 1984; cells were described in the literature to be EBV-negative, to carry the t(8;14) translocation effecting MYC-IGH and a TP53 point mutation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14427, 15026, 15976, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorph cells growing in clusters; image; image; image; image |
| Medium: | 80-90% Iscove's MDM + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml in flasks; maintain at ca. 0.5-3.0 x 106 cells/ml; split culture 1:3-1:5 every 2 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-24 hours |
| Harvest: | maximum density at ca. 3.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD10+, CD13-, CD19+, CD20+, CD34-,
CD37+, CD38+, CD80-, CD138-, HLA-DR+,
sIgG-, sIgM+, sIg-kappa+, sIg-lambda-, cIgG-,
cIgM+, cIg-kappa+, cIg-lambda-, cyCD79a+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 3% polyploidy; 46(42-48)<2n>XY, inv(6)(q12q21), t(8;14)(q24;q32), del(18)(q21.1); carries t(8;14) effecting MYC-IGH rearrangement; matches published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
