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| Cell line: | OE-33 |
| DSMZ no.: | ACC 706 |
| Species: | human (Homo sapiens) |
| Cell type: | esophageal adenocarcinoma |
| Origin: | established from the primary tumor of a 73-year-old woman with Barrett-esophageal adenocarcinoma in 1993; cells are described in the literature to produce tumors in nude mice; cell line also known as JROECL33; genome sequence data in ref 40712 and 40877 |
| Reference(s): | 16055, 16110, 40877, 18232, 40712 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently in single cells and clusters; image; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture up to 1:10 every 4-5 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertetraploid karyotype; 92-100<4n>XX, -X, -X, +1, -4, +5, +6, +7, +7, +7, +7, +8, +10, +10, -13, -13, -13, +16, +16, +16, -17, -18, -18, -19, +20, +20, +20, +20, -21, -22, -22, +6mar; add(1)(p13)x2, i(3)(q10)x1-2, del(5)(p14.2), add(6)(q22), add(7)(q34)x3, der(11)add(11)(q23)i(11)(q10), add(14)(p11) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
