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| Cell line: | OTH-74D4 |
| DSMZ no.: | ACC 478 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (CD45RA) |
| Origin: | described as secreting a mouse monoclonal antibody (IgG1) CD45RA; the cell line was established in 1985 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with an EBV-transformed B cell line from a patient with MHC class II deficiency |
| Reference(s): | 15170 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round, single cells growing in suspension and some slightly adherent; image; image; image |
| Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture: | seed out at 0.3 x 106 cell/ml; maintain at 0.2-0.5 x 106 cells/ml; split saturated cultures 1:2 to 1:3 every 2 days; HFCS can be used at 1:100 (i.e. half of the manufacturer recommendation) |
| Incubation: | at 37°C with 10% CO2 |
| Doubling time: | about 40-50 hours |
| Harvest: | maximal density of about 0.6-0.8 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | secretion of a IgG1 monoclonal antibody |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
