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| Cell line: | P-815 |
| DSMZ no.: | ACC 1 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | mast cell |
| Origin: | established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene; used as target cells for cytotoxic T cell assays; as reported cells exhibit no effector activity in an antibody-dependent cell mediated cytotoxic system |
| Reference(s): | 14875, 14876, 14877, 14874, 15207 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round cells in suspension; some cells may be adherent; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1 x 106 cells/ml; starting in a 12- or 24-well plate may be of advantage;
maintain at 0.25-1.5 x 106 cells/ml; cells grow very fast; split 1:4 to 1:8 every 3-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 18-22 hours |
| Harvest: | maximum cell density at > 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near-diploid karyotype - 41(36-43)<2n> - 1-2 centric fusion markers present |
| Molec. Genetics: | genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
