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Cell line: | P493-6 |
DSMZ no.: | ACC 915 |
Species: | human (Homo sapiens) |
Cell type: | B lymphoblastoid cells |
Origin: | genetically modified cell line established in 1994 from the EBV-positive B-lymphoblastoid cell line ER/EB2-5 (DSMZ ACC 914) by stable transfection with plasmid pmyc-tet, which also encodes the transactivator protein tTA. MYC expression is observed in the absence of tetracycline and is repressed upon its addition. |
Reference(s): | 40222, 40223, 40220, 40221 |
Biosafety level: | 2, GMO-S2 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2 according to the German Biological Agents Regulation. Furthermore, the cells are genetically modified (EBNA2 expression inducible by estrogen and MYC expression suppressible by tetracyclin), which renders the cells in combination with the EBV infection biosafety level 2 according to the German Act on Genetic Engineering. |
Permissions and restrictions: | A, B, D |
| DSMZ Cell Culture Data: |
Morphology: | mostly round cells growing in suspension; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS + 2mM L-glutamine (expansion of the cell culture in the absence of tetracycline is recommended) |
Subculture: | seed out 0.5 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 36 hours |
Harvest: | maximal density of ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD21 +, CD34 -, CD37 +, CD38 +, cy CD79a +, CD80 -, CD138 +, HLA-DR +, cy IgM +, cy IgG -, cy kappa +, cy lambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, otherwise identical to parental cell line ER/EB2-5 |
Molec. Genetics: | MYC supression by tetracyclin (0.1 µg/ml, 72 h) was confirmed by Western blot analysis |
Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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