| | |
|---|
| Cell line: | PA-TU-8902 |
| DSMZ no.: | ACC 179 |
| Species: | human (Homo sapiens) |
| Cell type: | pancreas adenocarcinoma |
| Origin: | established in 1985 from the primary ductal pancreatic adenocarcinoma (grade II) from a 44-year-old woman; reported as inducing metastasis in nude mice, to carry mutations in KRAS and TP53 and to secrete cytokines and proteinases |
| Reference(s): | 14509, 18168, 40710, 40728 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial adherent cells growing in monolayers; image; image; image; image; image |
| Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 0.5-2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 25-40 hours |
| Harvest: | cell harvest of 4-8 x 106 cells/80cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded pseudotetraploid karyotype - 91(83-94)<4n>XXX-X, +5, -6, -6, +7, -10, -10, +12, -13, -13, -14, -19, +20, -22, -22, +5mar, del(4)(q26)x2, der(5)t(5;14)(p11;q11), der(5)t(5;?)(q11;?), der(7)t(7;?)(p14;?), der(9)t(9;11)(p12;q11), der(14)t(?5;14)(?p11;p11)x2, del(17)(p12), der(17)t(17;?)(p11/12;?), der(18)t(18;?)(p12;?), der(19)t(19;?)(q13.1;?), ?i(21q)x3, der(22)t(22;?)(q13;?) - carries 19p13.1 recurrent tumor breakpoint - corresponds to vague published description |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
