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| Cell line: | PAB-122 |
| DSMZ no.: | ACC 166 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-p53) |
| Origin: | established by fusion of NS-1 myeloma cells and spleen cells from a male BALB/c mouse immunized with SV40-transformed, 3T3 clone 4 mouse cells; cells were reported to secrete an IgG2b antibody recognizing p53 protein present in nuclei of SV40-transformed cells |
| Reference(s): | 14583, 15206 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single cells in suspension; image; image |
| Medium: | 80% DMEM + 20% h.i. FBS |
| Subculture: | maintain at 0.5-5 x 105 cells/ml; split 1:5 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Harvest: | maximal density at 1-2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | ELISA: reverse transcriptase negative |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
