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established from the peripheral blood of a 4-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) in second relapse in 1977;
carries NUP214-ABL1 fusion gene; of note: PEER is genetically identical to cell line BE-13 (DSMZ ACC 396); however, PEER carries a diploid and BE-13 a tetraploid karyotype suggesting that BE-13 is a subclone of PEER, rather than vice-versa
cells are very difficult to culture! Cells grow at best in 24-well-plates; seed out at ca. 1.0 x 106 cells/ml (culture should not be split during the first 3-4 days after thawing), then maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days
at 37 °C with 5% CO2
ca. 50-60 hours
saturation density at 1.5 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays