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| Cell line: | PEER |
| DSMZ no.: | ACC 6 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell leukemia |
| Origin: | established from the peripheral blood of a 4-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) in second relapse in 1977;
carries NUP214-ABL1 fusion gene; of note: PEER is genetically identical to cell line BE-13 (DSMZ ACC 396); however, PEER carries a diploid and BE-13 a tetraploid karyotype suggesting that BE-13 is a subclone of PEER, rather than vice-versa |
| Reference(s): | 14574, 14608, 14884, 15867, 15868, 15217 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small, round cells growing mostly singly in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | cells are very difficult to culture! Cells grow at best in 24-well-plates; seed out at ca. 1.0 x 106 cells/ml (culture should not be split during the first 3-4 days after thawing), then maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | saturation density at 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD13 -, CD19 -, CD34 -, TCRgamma/delta +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an
authentic profile of the reference STR database. Note: cell line BE-13 shows an identical STR profile |
| Cytogenetics: | human pseudodiploid karyotype with 7% polyploidy - 46(42-47)<2n>XX, der(4)?dup ins(4;4)(?p11;?q21q25), del(5)(q22q31), del(6)(q13q22), del(9)(p11p22), del(9)(q22) - resembles published karyotype - 5q-, 6q-, 9p- and 9q- associated with hematological malignancy |
| Molec. Genetics: | expression of NKX2-5 and MEF2C confirmed by RT-PCR and Western blot and expression of the fusion gene NUP214-ABL1 by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
