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Cell line: | PR-1 |
DSMZ no.: | ACC 486 |
Species: | rat (Rattus norvegicus) |
Cell type: | pituitary tumor |
Origin: | established from the pituitary tumor of an ovarectomized F344 rat treated with estrogen for 3 months; described as showing markedly reduced levels of TGF-ß1 and TGF-ß type II receptor mRNA; reported as being resistant to antiproliferative effects of TGF-ß1 |
Reference(s): | 14835 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells, slightly adherent, growing as strings of round cells in branched colonies |
Medium: | DMEM + Ham's F12 (1:1) + 2.5% h.i. FBS + 10% horse serum |
Subculture: | optimal split ratio of 1:2 once a week using trypsin/EDTA; seed out at ca. 3 x 106 cells/80 cm2 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 90 hours |
Harvest: | cell harvest of ca. 50 x 106 cells/80 cm2 |
Storage: | frozen with 65% medium (1:1), 5% FBS, 20% horse serum, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
Cytogenetics: | rat near-triploid karyotype with 10% polyploidy - 60-68<3n> |
Viruses: | PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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