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| Cell line: | PSI-2 |
| DSMZ no.: | ACC 238 |
| Species: | murine (mouse, Swiss mouse) (Mus musculus) |
| Cell type: | embryo - NIH-3T3 derivative (retroviral packaging line) |
| Origin: | subclone of the Swiss mouse embryo cell line NIH-3T3; PSI-2 cells are infected with a replication deficient MLV virus; cells can be used as "packaging cell line"; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS) |
| Reference(s): | 14732 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent fibroblastoid cells growing as monolayers; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | split confluent culture 1:10 to 1:20 every 2-3 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 34 hours |
| Harvest: | cell harvest of about 2-4 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypotetraploid karyotype - centric fragment present at 74% - ring present at 48% - 68/69(64-74)<4n> - a submetacentric marker arising by centric fusion present at 52% |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
