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Cell line: | RCH-ACV |
DSMZ no.: | ACC 548 |
Species: | human (Homo sapiens) |
Cell type: | B cell precursor leukemia |
Origin: | established from bone marrow cells taken at relapse of common acute lymphoblastic leukemia (cALL), seven months after diagnosis, from an 8-year-old girl treated with combination chemotherapy |
Reference(s): | 14634, 14661 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | small round cells growing singly or in small clumps in suspension; occasional adherent cells present; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/ml; split saturated culture 1:2 to 1:10 every 2-6 days; maintain at ca. 0.5-2.5 x 106 cells/ml; easy to culture, tolerates low cell densities |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-50 hours |
Harvest: | cell harvest of ca. 4 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD13 -, CD19 +, CD37 -, cyCD79a +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hyperdiploid karyotype with 2% polyploidy - 43-50<2n>XX, +8, t(1;19)(q23;p13.3) - sideline with idem, +16, +der(19)t(1;19) - carries t(1;19) effecting TCF3-PBX1 (E2A-PBX) fusion - matches published karyotype |
Molec. Genetics: | expression of fusion gene TCF3-PBX1 (E2A-PBX) confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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