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Cell line: | RPMI-7951 |
DSMZ no.: | ACC 66 |
Species: | human (Homo sapiens) |
Cell type: | malignant melanoma |
Origin: | established from an involved lymph node of an 18-year-old woman with malignant melanoma in 1971 |
Reference(s): | 14872 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent, epithelial-like cells growing as monolayers; image; image; image |
Medium: | 90% DMEM + 10% h.i. FBS |
Subculture: | split confluent culture 1:4 to 1:10 every 4-6 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 60 hours |
Harvest: | about 10-12 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hyperdiploid karyotype with 22% polyploidy - 54(50-60)<2n>X, -X, +1, +2, +6, +7, +8, +20, +20, +3mar, der(8)t(7;8)(p11;p12), der(9)t(9;11)(p21;q11), add(18)(q23;HSR) - shares t(9;11) with IPC-298 defining a new recurrent translocation in malignant melanoma |
Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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