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| Cell line: | RS-5 |
| DSMZ no.: | ACC 604 |
| Species: | human (Homo sapiens) |
| Cell type: | sarcomatoid mesothelioma |
| Origin: | established in 2003 from the untreated malignant mesothelioma of the pleura of a 78-year-old man with asbestos cancer |
| Reference(s): | 14850 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, sarcomatoid-like cells growing in monolayers; image; image; image |
| Medium: | 90% NCTC-109 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:4 once a week using trypsin/EDTA; cells grow slowly for about one week after thawing |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 3 days |
| Harvest: | maximum density of ca. 3 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded near-diploid karyotype with 5% polyploidy; 46(39-48)<2n>XY; although no consistent abnormality was detected, most metaphases showed random chromosome loss |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
