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| Cell line: | RS4;11 |
| DSMZ no.: | ACC 508 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established from the bone marrow of a 32-year-old woman with acute lymphoblastic leukemia (ALL L2) in first relapse |
| Reference(s): | 14493, 14988, 15227, 15228 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, relatively small, round cells growing in suspension; image; image; image; image |
| Medium: | 80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at about 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | maximal density of about 4.0 x 106 cells/ml |
| Storage: | frozen in serum-free freezing medium (BambankerTM) |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 -, CD34 -, CD38 +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype - 47/48<2n>X/XX, +8, +18, t(4;11)(q21;q23), i(7q) - sideline with +8/18 - carries KMT2A-AFF1 (MLL-AFF1; MLL-AF4) fusion gene - resembles published karyotype |
| Molec. Genetics: | expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) e10-e4 confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
