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| Cell line: | RTG-2 |
| DSMZ no.: | ACC 352 |
| Species: | fish - rainbow trout (Oncorhynchus mykiss) |
| Cell type: | gonadal cells |
| Origin: | established from the gonadal cells of a rainbow trout (salmo gairdneri) |
| Reference(s): | 15067, 18247 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | long spindle-like and fibroblast-like cells growing adherently in monolayers; image; image; image |
| Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine + 50 mg/L neomycin sulfate |
| Subculture: | split subconfluent culture 1:2 every 5-7 days using trypsin/EDTA at cold temperature; seed out at ca. 0.5 x 106 cells/25 cm2; growth is inhibited at a pH higher than 7.4 |
| Incubation: | maximum temperature at 26 °C, optimal at 20 °C, grown with CO2 |
| Doubling time: | ca. 3-7 days |
| Harvest: | cell harvest of ca. 4 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization and PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Oncorhynchus mykiss species. |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
