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Cell line: | RTgill-W1 |
DSMZ no.: | ACC 899 |
Species: | fish -rainbow trout (Oncorhynchus mykiss) |
Cell type: | gill |
Origin: | established in 1990 from the gill of a juvenile rainbow trout; cells are used in OECD guideline Test No. 249 (Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay) for testing of acute toxicity of chemicals; cells are further described to
support growth of certain viruses and fish parasites |
Reference(s): | 18240, 18239, 18238, 18247 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | For approval of distribution to for-profit organizations please contact kristin.schirmer@eawag.ch |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently as monolayer; image; image; image |
Medium: | 95% Leibovitz´ L15 (without phenol red) + 5% (not inactivated) FBS |
Subculture: | cells grow very slowly; seed out 1-1.5 x 106 cells in a 25 cm2 flask; split confluent culture 1:2 every 10-14 days as follows: rinse 2x with 1ml versene (0.2 g/l) add 0.6-0.8 ml trypsin (0.25% (for 1-2 min)) and detach carefully; avoid sheering the cells or formation of foam; change medium every 5-7 days |
Incubation: | at ca. 20°C in the dark, without CO2 gas exchange |
Doubling time: | ca. 10-12 days |
Harvest: | cell harvest of 8-10 x 106 cells/80 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with MRA and BM-cyclin by the originator, then proofed as negative by PCR at the DSMZ |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Oncorhynchus mykiss species. |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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