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| Cell line: | RV-C2 |
| DSMZ no.: | ACC 289 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-rat fiber/troponin) |
| Origin: | described as secreting a mouse monoclonal antibody (IgG2b) reacting with fetal, neonatal and adult cardiac fibers in immunofluorescence and with rat cardiac fetal and adult troponin T in Western blotting; in immunofluorescence this antibody stains some rat fetal and neonatal skeletal muscle fibers which express fetal cardiac troponin T as also shown by the Western blotting of rat fetal and neonatal skeletal muscle; this cell line was produced by fusing NSO myeloma cells with spleen cells from a Balb/c mouse that had been immunized with a myofibrillar preparation from hypothyroid rabbit ventricle |
| Reference(s): | 14910, 14908 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round single cells in suspension; image; image; image |
| Medium: | 80% DMEM (4.5 g/L glucose) + 20% horse serum + 2 mM L-glutamine |
| Subculture: | optimal split ratio of 1:2 to 1:3 twice a week; seed out at about 0.5-1.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 32 hours |
| Harvest: | maximal density at about 2.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% horse serum, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | secretion of a IgG2b monoclonal antibody |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypertetraploid/pentaploid karyotype - 88-99<4n> - 2 centric fusion markers and 1-2 dmin present in most cells |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
