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| Cell line: | SCC-25 |
| DSMZ no.: | ACC 617 |
| Species: | human (Homo sapiens) |
| Cell type: | squamous cell carcinoma |
| Origin: | established from the biopsy of a squamous cell carcinoma of the tongue of a 70-year-old man; described to carry a TP53 mutation |
| Reference(s): | 14889, 40425 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently in monolayers; image; image; image |
| Medium: | 80% mixture of Ham's F12 + DMEM (at 1:1) + 20% h.i. FBS, 2 mM L-glutamine, 1 mM sodium pyruvate |
| Subculture: | seed out at ca. 1.0 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA (wash twice with PBS before trypsination) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | maximum density at ca. 5 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human highly rearranged hypertriploid karyotype with 4% polyploidy; 72-82<3n>XX, -Y, +4, -7, +9, +9, -13, -14, +15, -18, -19, -21, +10, -12 mar; add(1)(p31)x2, add(3)(?p11), add(4)(q11), i(5p), i(5q), der(8)t(1;8)(q25;p11)x1-2, del(9)(p11), i(9q), add(11)(q25), i(13q), add(14)(p11), add(15)(p11)x3-4, add(21)(q22) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
