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Cell line: | SCHNEIDER-2 |
DSMZ no.: | ACC 130 |
Species: | insect - fruitfly (Drosophila melanogaster) (Drosophila melanogaster) |
Cell type: | |
Origin: | established from the late embryo of a Drosophila melanogaster (fruitfly) in 1972; originally the cells were diploid with 5-10% XY, currently the cell line has only XX cells that are now 60-80% tetraploid; also often termed (incorrectly) S2 |
Reference(s): | 14943 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | small adherent cells growing in monolayers; a small number of cells is also in suspension; image; image; image |
Medium: | mix of Schneider's Drosophila medium + TC-100 medium (1:1) + 10-20% h.i. FBS |
Subculture: | maintain at 24-30°C with gas exchange; seed out at ca. 1-2 x 106 in T25 flask; after thawing cells may have a long lag period (3-7 days) before they start to grow; split confluent culture 1:2 to 1:4 every 2-4 days; cells are resistant to trypsination, for propagation use cells in suspension or remove adherent cells with a rubber policeman |
Incubation: | at room temperature up to 30 °C in normnal atmosphere |
Doubling time: | ca. 40-60 hours |
Harvest: | about 15-20 x 106 cells in a 25 cm2 flask |
Storage: | frozen with 35% Schneider´s Drosophila medium, 35% TC-100 medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Drosophila melanogaster |
Viruses: | PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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