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| Cell line: | SD-1 |
| DSMZ no.: | ACC 366 |
| Species: | human (Homo sapiens) |
| Cell type: | peripheral blood B lymphoblastoid cells (from patient with acute lymphoblastic leukemia) |
| Origin: | established from the peripheral blood of a woman with acute lymphoblastic leukemia (pre B-ALL) at diagnosis; cells were immortalized with EBV; shown at DSMZ by RT-PCR to carry minor breakpoint BCR-ABL1 fusion for which it is a suitable positive control for RT-PCR |
| Reference(s): | 14484, 14458 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | ovoid to round cells growing in suspension, singly or in clumps; image; image; image; image |
| Medium: | 85-90% RPMI 1640 + 5-10% h.i. FBS |
| Subculture: | seed out at ca. 0.3 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split cells 1:3 to 1:5 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 28-32 hours |
| Harvest: | reaches maximum density of ca. 1.3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3-, CD10-, CD13-, CD19+, CD20+, CD34-, CD37+, CD38-, CD80+, CD138(+), HLA-DR+, cyCD79a(+), sIgG+, cIgG+, sIgM-, cIgM-, sIg-kappa+, cIg-kappa+, sIg-lambda-, cIg-lambda-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-tetraploid karyotype - 92(88-92)<4n>XXXX, t(9;22)(q34;q11)x2 - carries two balanced Ph translocations - tetraploid derivate of original diploid karyotype |
| Molec. Genetics: | expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
