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| Cell line: | SGE-1 |
| DSMZ no.: | ACC 343 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | kidney glomerular epithelial |
| Origin: | established from isolated renal glomeruli of a 3-week-old normal male Wistar rat in 1988 |
| Reference(s): | 15068, 15069 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells growing as monolayer with polygonal shapes; image; image; image |
| Medium: | 45% DMEM + 45% Ham's F12 + 10% h.i. FBS + 1x insulin-transferrin-sodium selenite + 10 ng/ml EGF |
| Subculture: | split culture prior to confluency, 1:10 to 1:20 twice a week using trypsin/EDTA; seed out at ca. 2-2.5 x 104 cells/cm2; cells grow also with coating of the flask with collagen |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 33 hours |
| Harvest: | cell harvest of ca. 20 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat flat-moded hyperdiploid karyotype - 43-48<2n> |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
