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| Cell line: | SIG-M5 |
| DSMZ no.: | ACC 468 |
| Species: | human (Homo sapiens) |
| Cell type: | acute monocytic leukemia |
| Origin: | established in 1995 from the bone marrow of a 63-year-old man with acute myeloid leukemia of monocytic origin (AML FAB M5a) at diagnosis |
| Reference(s): | 15049 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | large round cells growing singly in suspension; image; image; image; image |
| Medium: | 80% Iscove's MDM + 20% h.i. FBS |
| Subculture: | cells may be difficult to culture; seed out at ca. 1 x 106 cells/ml; split saturated culture 1:2 every 3 days; after thawing, it is advisable to initially grow the cells in 12- or 24-well plates; cell viability remains continuously at or below 70% |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 72 hours |
| Harvest: | maximum density of about 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3-, CD4-, CD13-, CD14(+), CD15+, CD19-, CD33+, CD34-, HLA-DR-, cyCD68-, cyCD3-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypertriploid/hypotetraploid karyotype with 4% polyploidy - 78(77-81)<3n>XX/XXY, +1, +2, +3, +4, +5, +8, -9, -10, +12, +15, +16, +17, +18, +21, +22, +0-2mar - i(1q), add(17)(p1?1) - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
