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| Cell line: | SISO |
| DSMZ no.: | ACC 327 |
| Species: | human (Homo sapiens) |
| Cell type: | cervix adenocarcinoma |
| Origin: | established from the surgically removed, chemotherapy-resistant cervix adenocarcinoma of a 67-year-old woman (clinical stage FIGO Ib) |
| Reference(s): | 15160 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial growth in compact colonies; cells tend to pile up after confluency; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | split confluent culture 1:5 to 1:10 every 5-7 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/175 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 35 hours |
| Harvest: | cell harvest of ca. 40 x 106 cells/175 cm2 (ca. 2.3 x 105 cells/cm2) |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | original contamination was eliminated with Ciprobay (ciprofloxacin), thereafter negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 12% polyploidy - 61<3n>XXX, -1, -2, -3, -4, -6, -8, -10, -13, -14, -16, -17, -19, -22, +4mar, add(X)(q28), add(5)(p15), add(6)(p23), del(9)(q34), add(11)(q24), del(12)(q15), der(12)t(12;17)(p12;q11), add(13)(p12), add(18)(q23)x1-2 - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
