| | |
|---|
| Cell line: | SK-HEP-1 |
| DSMZ no.: | ACC 141 |
| Species: | human (Homo sapiens) |
| Cell type: | liver adenocarcinoma |
| Origin: | established from the ascites of a 52-year-old man in 1971 (with hepatocellular carcinoma) |
| Reference(s): | 14537 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing as monolayer; image
; image; image
; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 3-4 x 106 cells/80 cm2; split confluent culture 1:10 to 1:20 about 1-2 times a week using trypsin/EDTA; cells grow fast |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | about 15-18 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization and PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 9% polyploidy - 62(60-63)<3n>X, -X, -X, -4, +6, +7, -9, +12, -13, -14, -15, -16, -18, der(1;7)(p10;q10), ?der(2;20)(p10;q10), der(6;13)(p10;q10)x2-3, add(12)(p1?), i(14q), add(19)(p13), add(21)(p11) - sideline with add(15)(p11) - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
