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| Cell line: | SK-N-MC |
| DSMZ no.: | ACC 203 |
| Species: | human (Homo sapiens) |
| Cell type: | Askin tumor |
| Origin: | established from the supraorbital lymph node metastasis of an Askin tumor (originally diagnosed as neuroblastoma) of a 14-year-old girl in 1971 (see ATCC HTB 10); although described in the original paper as neuroblastoma, SK-N-MC is now widely regarded as having originated from the morphologically similar Askin's tumor related to Ewing's sarcoma; FLI1-EWS gene fusion, the molecular pathological change in Ewing's sarcoma and Askin's tumor, was shown in SK-N-MC by Dunn et al. |
| Reference(s): | 14976, 14388, 15225 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblast-like cells growing adherently as monolayer; image; image; image; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids |
| Subculture: | split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA; seed out at ca. 1-4 x 106 cells/80 cm2; cells may grow slowly initially |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | cell harvest of about 25 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human pseudodiploid karyotype with 6% polyploidy - 46(40-47)<2n>XX, +8, -16, t(1;4)(p36;p13), t(2;3)(q24;q29), der(3)t(3;3)(p13;q22), del(10)(q25), t(11;22)(q24;q12), t(15;22)(q21;q12), add(17)(p12), add(21)(p12) - dmin in 9% of cells - resembles published karyotype - carries masked 11;22 translocation recurrent in Ewing's and Askin's tumor |
| Molec. Genetics: | expression of fusion gene EWS-FLI (type 1) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
