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| Cell line: | SKM-1 |
| DSMZ no.: | ACC 547 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established from the peripheral blood of a 76-year-old man with acute monoblastic leukemia (AML M5) in 1989 following myelodysplastic syndromes (MDS) |
| Reference(s): | 14796, 14797, 14798, 18138 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 15-20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; maintain at ca. 0.4-2.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | cell harvest of ca. 2.5 x 106 cells/ml |
| Storage: | frozen in serum-free freezing medium (BambankerTM) |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD4(+), CD13(+), CD14-, CD15(+), CD19-, CD33+, CD34(+), HLA-DR(+); image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 8% polyploidy - 43(38-43)<2n>XY, +1, -12, -14, -20, -21, t(1;19)(q21;q13), del(2)(p11), del(9)(q12), add(17)(p1?) - sideline with idem, der(10)t(10;?21)(p11;q11), -21 - consistent with published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
