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| Cell line: | SKW-3 |
| DSMZ no.: | ACC 53 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell leukemia (derivative of KE-37) |
| Origin: | originally described in the literature to be established from the peripheral blood of a 61-year-old man with T cell chronic lymphocytic leukemia (CLL) in 1977; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line KE-37; KE-37 was established from a 27-year-old man with acute lymphoblastic leukemia (ALL) in 1979 |
| Reference(s): | 14610, 14473 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round cells in suspension; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | saturation density at about 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, PCR assays |
| Immunology: | CD2+, CD3-, CD4+, CD5+, CD6+, CD7+,CD8+, CD13-, CD19-, CD34-, TCRalpha/beta-, TCRgamma/delta-, cyCD3+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near diploid karyotype with 4% polyploidy - 46(43-48)<2n>XY, +8, -14, t(3;3)(q11;q27), der(8)t(8;14)(q24;q11)x2, t(8;11)(p21;p12), der(12)t(12;?)(q24;?), der(14)t(8;14)(q24;q11) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
