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Cell line: | SU-DHL-5 |
DSMZ no.: | ACC 571 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established from a lymph node of a 17-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse large cell, noncleaved cell type; assigned to GCB-like lymphoma subtype (germinal center B-cell) |
Reference(s): | 14605, 15065, 14515, 17354, 14516 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | small, round to polymorph cells growing mostly in clumps; image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | initially seed out at ca. 0.5 x 106 cells/ml in a 24-well-plate (the viability may drop below 30% after thawing); later, maintain at ca. 0.1-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 2-3 days; note: viability does usually not exceed 85% |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40 hours |
Harvest: | cell harvest of ca. 1.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +
; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 1.5% polyploidy - 47(41-48)<2n>XX, +12, del(6)(q13), del(12)(q13) - sideline with del(6)x2 - matches published karyotype |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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