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Cell line: | SW-403 |
DSMZ no.: | ACC 294 |
Species: | human (Homo sapiens) |
Cell type: | colon adenocarcinoma |
Origin: | established from the adenocarcinoma (grade III) of a 51-year-old woman; cells were described in the literature to produce carcinoembryonic antigen (CEA) and to be tumorigenic in nude mice |
Reference(s): | 14713 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent round cells, sometimes aggregated in complexes of 20-100 cells; image; image |
Medium: | 90% DMEM + 10% h.i. FBS |
Subculture: | split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA; seed out at about 2.5-3.0 x 106 cells/25 cm2 |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | about 40-50 hours |
Harvest: | cell harvest of about 40 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypotriploid karyotype with 12% polyploidy - 62(58-70)<3n>XX/XXX, +2, +2, -8, -9, -10, -10, -12, -13, -14, -18, -19, -22, +mar, der(1)t(1;1)(p21;q22), del(4)(q21), add(5)(p15), del(6)(q24-q25), i(8q), del(9)(p21), i(9q), add(13)(p13), add(14)(p13) - carries very large isochromosome-marker, does not correspond to published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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